The ge hiload 1660 superdex 200 prep grade is a prepacked xk column for preparative gel filtration chromatography separations. Hiload 16600 and 26600 superdex 30 prep grade hiload. To obtain the full translocon complex a fivefold molar excess of im9 was added to the btuba33c cole9ompfp201c tolb. Hiload superdex 75 prep grade data file hiload superdex 200. Figure 10 shows mousemonoclonal igg 2b directly from cell supernatant. Chromatographic behavior of bothrops erythromelas phospholipase and other. Superdex prep grade is a preparative size exclusion chromatography resin with a composite matrix of dextran and agarose. Hiload 16600 superdex 200 prep grade are prepacked xk columns designed for highresolution preparative gel filtration chromatography. New superdex 200 increase columns product description ge cat. Ge healthcare size exclusion chromatography superdex 75. Hitrap heparin, 1ml amersham biosciences chemicals. Superdex 200 prep grade is recommended for the purification of monoclonal antibodies.
Regenerate the column after each run with one column volume of start buffer at. Superdex 30 prep grade medium is optimized for proteinspeptides below m r 10 000. Charge ion exchange chromatography iex hydrophobicity hydrophobic i nteraction chromatography hic reversed phase chromatography rpc biorecognition ligand specificity affinity chromatography ac gel filtration hydrophobic interaction ion exchange affinity reversed phase fig. Ge healthcare hiload 16600 superdex 200 pg promotion. The steep selectivity of dextran and the high chemical and physical stability of agarose enable high resolution separations. Hiload 16600 superdex 200 pg ge healthcare, 28989335.
Calibration of hiload 2660 superdex 200 prep grade. Superdex 200 increase from, your source for laboratory equipment and scientific supplies. Gel filtration john curtin school of medical research. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose.
Highsample load 500 l separation of a mixture containing seven. Protocol for the purification of wnt proteins the wnt homepage. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments 1660 superdex 200 prep grade is a prepacked xk column for preparative gel filtration chromatography separations. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range mr 10 000 600 000 superdex 200 pg. Superdex 200 prep grade ge healthcare life sciences. Size exclusion chromatography columns and media ge. Superdex 200 prep grade pg are prepacked xk columns designed for preparative size exclusion chromatography. New agarose based size exclusion chromatography sec columns methods for. New superdex 200 increase columns fisher scientific. Pdf chromatographic behavior of bothrops erythromelas. Hiload 1660 or 2660 superdeprep grade hiload 1660 or 2660 superdex 75 prep grade hiload 1660 or 2660 superdex 200 prep grade dismantling tool, support screens, net rings.
Two novel gel filtration media, superdex 75 prep grade and superdex 200 prep grade are described. These versatile columns offer rapid separations with high resolution for a variety of applications. Elution volumes for globular proteins of various molecular weights on superdex 75 increase 10300 blue, superdex 200 increase 10300 green, and superose 6 increase 10300 orange. Sephadex g 200, which is appropriate for separation of molecules in the 5,000 to 250,000 da range see note 5. Pressure vs flow velocity for superdex 200 prep grade in bpg 200 at 82. This combination of prepacked convenience and reproducibility make hiload superdex prep grade columns a confident choice for fast, highresolution gel filtration at preparative. The separation properties of these media are determined by the dextran componentchromatography columns separate molecules within mixtures during gas or liquid methods. Superdex 75 pg, use 200 mm sodium acetate in 20% ethanol. Superdex 75 prep grade and superdex 200 prep grade. Hiload 1660 or 2660 superdex 30 prep grade transport syringe hiload 1660 or 2660 superdex 75 prep grade hiload 1660 or 2660 superdex 200 prepgrade equilibration before a new run. Separation of test substances on hiload 26600 superdex 30 pg. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required.
Hiload superdex 200 prep grade hiload superdex 200 prep grade has separationrange 0600 000 highestselectivity between 30 000 250000. Protein purification chromatography calibration of hiload. Superdex prep grade is a preparative size exclusion chromatography resin. Selectivity curves from superdex 30 pg, superdex 75 pg, and. Superdex gel filtration media are listed in appendix c, table 1. Superdex prep grade is a composite matrix of dextran and. Polysaccharide leakage from superdex 30, 75 and 200 prep grade and sepharose hp in static experiments over a period of 4 weeks in different acidic conditions incubation solution hci 0001 m 001 m 01 m superdex 30 prep grade mgml 062 092 55 superdex 75 prep grade mgml 050 070 2. Hiload 1660 or 2660 superdex 200 prep grade amersham biosciences cation exchange column. Performance of superdex 200 prep grade measured as k av values of a protein mixture after repeated treatment with a 0. Three types of superdex prep grade are available in hiload columns. Each column is expertly packed and individually tested.
With their high physical and chemical stability, very high batchtobatch reproducibility, and regulatory support file backup, superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale operation from research and. To prevent any nonspecific interactions with the resins, it is recommended to use an eluent with an ionic. Dextran component determined the separation properties of these media. Bioprocess resin supported for industrial applications and wellestablished in approved processes. Stability superdex pg can be used in aqueous solutions with a ph range of 3 to12 for continuous operation, and ph 1 to 14 for cleaninginplace cip. Hiload superdex 200 preparation grade resin is well suited for rapid, preparative protein purification and scaleup. Hiload superdex 200 prep grade 12 index of references3 ref 18115696 edition ab, 200204 21. Hiload superdex 30, 75, and 200 pg columns bring convenience and high resolution to gel filtration. Hiload superdex 75 prep grade data file hiload superdex. They save time when screening many samples, and require less buffer and sample than longer columns. Superdex 200 increase prepacked columns are designed for size exclusion chromatography sechigh resolution gel filtration in smallscale g to mg, preparative purification, as well as for characterisation and analysis of proteins with molecular weights mr from 10 000 to 600 000. Characteristics of superdex prep grade media for gel.
Hiload superdex 30, 75, and 200 pg columns bring convenience and high resolution to size exclusion chromatography. Superdex 75 10300 gl and superdex 200 10300 gl are tricorn high performance columns. Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. Twostep purification of histidinetagged membrane proteins. Ge healthcare hiload 16600 superdex 200 pg hiload 1660. Hiload superdex columns, 200 pg, ge healthcare vwr. Gel filtration chromatography columns, superdex 200 increase.
For use on an fplc system, as discussed here, a superdex 200 prep grade column is recommended fractionation range of 10,000 to 600,000 da. Hiload superdex 200 prep grade are prepacked xk columns designed for highresolution preparative gel filtration chromatography. Hiload superdex 200 pg preparative sec columns cytiva. The protein was then loaded on a superdex 200 prep grade gelfiltration column pharmacia equilibrated with buffer c 50 mm trishcl ph 7.
Polysaccharide leakage from superdex 30, 75 and 200 prep grade and sepharose hp in static experiments over a period of 4 weeks in different acidic conditions incubation superdex 30 superdex 75 superdex 200 sepharose hi solution prep grade prep grade prep grade mgml hci mgml mgml mgmo 0001 m 062 050 009 002 001 m 092 0. Artisan technology group is your source for quality. Superdex prep grade pg is a preparative gel filtration chromatography. Instructions 56119099 ae gel filtration media superdex 30. The new media have high selectivities similar to those of sephadex g75 and g200, respectively, but permit the use of high flowrates as with crosslinked agarose hbeads.
Life sciences gel filtration columns and media selection guide imagination at work. Chemical, physical and chromatographic properties of superdex. Superdex 200 prep grade pg are prepacked xk columns designed for preparative gel filtration. A major advantage of the superdex 200 prep grade chromatography medium is. Its selectivity curve gives a broad fractionation range from m r 10 000 to 600 000, with excellent resolving power for antibody monomers, dimers and contaminants of lower molecular weight, such as albumin and transferrin. Comments the gel filtration step removes most of remaining contaminants as well as imidazole used for elution from the histrap ff crude column.
Promo code superdex 200 increase 10300 gl suitable for both smallscale mg, preparative and highresolution analysis applications 28990944 45002570 45002570pr superdex 200 increase 5150 gl designed for rapid purity checking and quick screening of different. Superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. Instructions for hiload 1660 superdex 200 pg column code no. Xk 5060 superdex 200 pg ge healthcare life sciences.
Superdex prep grade is a composite matrix of dextran and crosslinked agarose. Hiload superdex 75 prep grade 12 index of references3 ref 18115695 edition ab, 200204 51. A 2 mg in analytical run on a superdex 200 hr 1030 column. Superdex 200 increase columns superdex 200 increase prepacked columns fig 1 are designed for size exclusion chromatography sechigh resolution gel filtration in smallscale g to mg, preparative purification, as well as for characterization and analysis of proteins with molecular weights m r from 10 000 to 600 000. Hiload 16600 and 26600 superdex 30 prep grade hiload 16. Fractions containing heptameric mhsp10 were concentrated to.
The column is an excellent choice for separating mabs from dimers and contaminants. A gel filtration separation can be performed in the presence of essential ions or cofactors, detergents, urea, at high or low ionic strength, at 37 c or in the cold room according to the requirements of the experiment. Superdex prep grade is a composite matr ix of dextran and highly crosslinked agarose. Bpg 200 superdex 30 prep grade bpg 200 superdex 200 prep grade bpg 200 superdex 75 prep grade fig. Selectivity curves from superdex 30 prep grade, superdex 75 prep grade, and superdex 200 prep grade. As shown in figures 4 a and b, the detection signal from the run with superdex 200 increase 3. All are prepacked to 600 mm bed heights in 16 mm or 26 mm diameter columns. Pdf structural characterization of vapb46 antitoxin from. The new media have high selectivities similar to those of sephadex g75 and g 200, respectively, but permit the use of high flowrates as with crosslinked agarose hbeads. Superdex 30, 75 and 200 prep grade are preparative gel filtration media with a unique composite matrix of dextran and agarose. For olecules with molecular weights between 0 and 600000 da.
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